Dispase II(Neutral protease,grade II)dispase酶

Application

Dispase is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid and effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.

Product Description

EC 3.4.24.4
Enzyme activity: ≥0.8  U/mg (37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg²⁺, other heavy metals. Dispase is not inhibited by serum.
Specificity: Dispase is a nonspecific metalloprotease.
Working concentration: 0.6 to 2.4 U/ml

Quality

Non-sterile lyophilizate

Benefits

• Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
• Maintains cell membrane integrity
• Non-mammalian source - free of mycoplasma and animal virus contamination
• Extremely stable to influences of temperature, pH, and interference by serum components
• Easily inactivated by chelating agents or by dilution
• Delivers higher activity and convenience

Contents

5 vials with 1 g each; non-sterile lyophilizate

Preparation of stock and working solution:
Dissolve the non-sterile lyophilized enzyme in PBS (Ca²⁺ /Mg²⁺-free) (10 mg/ml). Dilute further with PBS (Ca²⁺ /Mg²⁺-free) to a final concentration of 0.6 to 2.4 U/ml. Concentrations higher than 2.4 U/ml are not recommended. Filter sterilize through a 0.22 μm filter membrane.

Background Information

One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine in 1 minute under assay conditions.  A practical comparison of Roche units of Dispase^? II with those cited in the Japanese literature (where dispase concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.