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Arachis hypogaea (PNA, Peanut Agglutinin) (FITC)/花生凝集素(PNA),FITC标记

产品编号:A3305-20
产品规格:2mg
价  格:3927.00
供 应 商:USbiological
库  存:常年库存
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Specifications:

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of approximately 27kD each, is galactose-specific, with strong anti-T-antigen activity. T-antigen") is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells. The presence of Ca++ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies.

Carbohydrate Specificity:
Terminal b-Galactose

Inhibitory:
Lactose > b-Galactose.

Activity:
<1ug/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Important Note: Protect FITC conjugate from light. by performing protocol in dark room or with foil covering where possible.

Molecular Weight:
120kD

Source: peanut


Concentration: ~1mg/ml


FITC:Protein Ratio: 1-2


Form: Supplied as a liquid in 0.02M sodium bicarbonate, pH 9.0-9.5, sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Newman, R.A., Hoppe-Seyler’s Z. Physiol. Chem. 358: 1517 (1977).

2. Novogrodsky, et al., J. Immunol. 115: 1243 (1995).

3. Pereira, et al., J. Exp. Med. 143: 422-436 (1975).

4. Lotaan, et al., Biochem. Biophys. Res. Comm. 62: 144 (1975).

5. Irimura, et al., Carbohydrates Res. 39: 317-327(1975).

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